Hb Lepore-Hollandia	deltabeta hybrid (delta through 22; beta from 50)

HEMATOLOGY		Mild anemia with microcytosis and hypochromia in the heterozygote (beta+-thal)
ELECTROPHORESIS		Hb X moves slower than Hb A at alkaline pH
CHROMATOGRAPHY		Hb X and Hb A can be separated by CM-cellulose or DEAE-Sephadex chromatography
STRUCTURE STUDIES		Tryptic digestion; separation of peptides by fingerprinting; amino acid analysis; sequencing
DNA ANALYSES		A large deletion was observed by gene mapping
FUNCTION STUDIES		Increased oxygen affinity; normal Bohr effect and cooperativity
STABILITY		Stable
OCCURRENCE		Found in several Papuan families
OTHER INFORMATION		Quantity in the heterozygote 12%; in the homozygote 26%

REFERENCES
1.		Barnabas, J. and Muller, C.J.: Nature, 194:931, 1962.
2.		Curtain, C.C.: Austrl. J. Exper. Biol. Med. Sci., 42:89, 1964.
3.		McDonald, M.J., Noble, R.W., Sharma, V.S., Ranney, H.M., Crookston, J.H., and Schwartz, J.M.: J. Mol. Biol., 94:305, 1975.

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This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.