Hb F-Veleta Ggamma40(C6)Arg->Gly
ELECTROPHORESIS Hb FX separates well from Hb A and Hb F; Hb X moves between Hb A and Hb F
IEF Hb FX focuses between Hb A and Hb A1
CHROMATOGRAPHY Hb FX elutes ahead of Hb F1 and Hb FO by cation exchange HPLC
CHAIN SEPARATION AgammaT = 14.1%; GgammaX = 33.4%; Ggamma = 35.5%; Agamma = 17%
DNA ANALYSES An AGG->GGG mutation at codon 40
NOTES Found in a Spanish newborn, who was also heterozygous for the AgammaT chain; family studies indicated that both mutations occur on the same chromosome (Agamma*Ggamma*/AgammaT*GgammaX*)
1. de Pablos Gallego, J.Ma., Gu, L-H., Leonova, J.Ye., and Huisman, T.H.J.: Hemo-globin, 19:407, 1995.

This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.