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ELECTROPHORESIS | |
Not reported | |
IEF | |
Hb FX moves between Hb F and Hb A | |
CHROMATOGRAPHY | |
Not reported | |
CHAIN SEPARATION | |
GgammaX = 35.9%; Ggamma = 33.5%; Agamma = 30.6% | |
STRUCTURE STUDIES | |
Tryptic digestion of gammaX chain; separation of peptides by fingerprinting; amino acid analysis; carboxypeptidase A | |
DNA ANALYSES | |
Not reported; presumed mutation CAC->TAC at codon 146 | |
NOTES | |
Found in a Japanese baby; Hb FX 26.4% of total Hb or 35% of total Hb F; increased oxygen affinity; normal Bohr effect and cooperativity | |