Hb F-M-Osaka Ggamma63(E7)His->Tyr
ELECTROPHORESIS No variant detectable
IEF No variant detectable
CHROMATOGRAPHY A broad Hb FX band elutes ahead of Hb A and Hb F on DEAE-cellulose chromatography
CHAIN SEPARATION GgammaX = 17.5% of total gamma; Ggamma = 40%; Agamma = 42.5%
STRUCTURE STUDIES Tryptic digestion of GgammaX; separation of peptides by reversed phase HPLC; amino acid analysis
DNA ANALYSES Not reported; presumed mutation CAT->TAT at codon 63
NOTES Found in a Japanese newborn and a Caucasian newborn; variant has a decreased oxygen affinity, increased Bohr effect; babies are cyanotic at birth; methemoglobinemia
1. Hayashi, A., Fujita, T., Fujimura, M., and Titani, K.: Hemoglobin 4:447, 1980.
2. Glader, B.E., Zwerdling, D., Kutlar, F., Kutlar, A., Wilson, J.B., and Huisman, T.H.J.: Hemoglobin, 13:769, 1989.

This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.