Hb F-La Grange	Ggamma101(G3)Glu->Lys

ELECTROPHORESIS		Hb FX moves slightly faster than Hb S
IEF		Not reported
CHROMATOGRAPHY		Hb FX can readily be isolated by DEAE-cellulose chromatography
CHAIN SEPARATION
STRUCTURE STUDIES		Tryptic digestion of gammaX chain; separation of peptides by reversed phase HPLC; amino acid analysis
DNA ANALYSES		Not reported; presumed mutation GAG->AAG at codon 101
NOTES		Found in a Caucasian newborn; Hb FX 14% of total Hb; variant forms hybrid Hbs with Hb F or Hb A; mildly unstable; increased oxygen affinity; lower heme-heme interaction

REFERENCES
1.		Nakatsuji, T., Shimizu, K., and Huisman, T.H.J.: Biochim. Biophys. Acta, 789:224, 1984.

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This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.