Hb F-Catalonia Ggamma15(A12)Trp->Arg
ELECTROPHORESIS Hb FX moves slower than Hb F (between Hb S and Hb C) at alkaline pH
IEF Hb FX focuses between Hb S and Hb C
CHROMATOGRAPHY Hb FX can readily be separated from Hb A and Hb F by DEAE-cellulose chromatography
CHAIN SEPARATION GgammaX = 36.8%; Ggamma = 35.4%; Agamma = 27.8%; also see NOTES
STRUCTURE STUDIES Tryptic digestion of gammaX chain; separation of peptides by reversed phase HPLC; amino acid analysis
DNA ANALYSES Not reported; presumed mutation TGG->CGG at codon 15
NOTES Found in two newborn babies from Northern Spain; the sec-ond baby was also an AgammaT heterozygote (Agamma75 Ile->Thr): AgammaT 11.5%; GgammaX 45.5%; Ggamma 31.3%; Agamma 11.7%
1. Plaseska, D., Wilson, J.B., Kutlar, F., Font, Ll., Baiget, M., and Huisman, T.H.J.: Hemoglobin, 14:511, 1990.

This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.