Hb F-Auckland Ggamma7(A4)Asp->Asn
ELECTROPHORESIS Hb FX moves slower than Hb F (between Hb S and Hb C) at alkaline pH
IEF No information
CHROMATOGRAPHY Hb FX was isolated by anion exchange HPLC
CHAIN SEPARATION GgammaX moves like Ggamma
STRUCTURE STUDIES Tryptic digestion of gammaX chain; separation of peptides by fingerprinting or reversed phase HPLC; amino acid analysis; sequencing
DNA ANALYSES Not reported; presumed mutation GAC->AAC at codon 7
NOTES Observed in newborns in New Zealand and in Alabama, USA; Hb FX ~19% of total Hb and 22.5% of total Hb F (DEAE-cellulose chromatography)
1. Carrell, R.W., Owen, M.C., Anderson, R., and Berry, E.: Biochim. Biophys. Acta, 365:323, 1974.
2. Chen, S.S., Wilson, J.B., Webber, B.B., Kutlar, A., and Huisman, T.H.J.: Hemoglobin, 9:531, 1985.

This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.