Hb A2-Flatbush delta22(B4)Ala->Glu
CONTACT External
ELECTROPHORESIS Hb X moves in the position of Hb S at alkaline pH
IEF Same
CHROMATOGRAPHY Hb X can be isolated by DEAE cellulose chromatography; Hb X elutes after Hb A2
STRUCTURE ANALYSIS Tryptic digestion of deltaX chain; separation of peptides by cation exchange HPLC; amino acid analysis; sequencing
DNA ANALYSIS Not reported; presumed mutation GCA->GAA at codon 22
NOTES Found in a few Black families
1. Ranney, H.M., Jacobs, A.S., Bradley, T.B., and Cordova, F.A.: Nature, 197:164, 1963.
2. Lee, R.C. and Huisman, T.H.J.: Blood, 24:495, 1964.
3. Jones, R.T., Brimhall, B., and Huisman, T.H.J.: J. Biol. Chem., 242:5141, 1967.

This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.