Hb A2-Canada delta99(G1)Asp->Asn
CONTACT Internal; near cavity; alpha2beta1 contact
ELECTROPHORESIS Hb X moves slower than Hb A2 at alkaline pH; Hb X cannot be separated from Hb B2 (delta16)
IEF Not reported
CHROMATOGRAPHY Hb X was isolated by DEAE-cellulose chromatography; Hb X elutes ahead of Hb A2
STRUCTURE ANALYSIS Tryptic digestion of AE-deltaX chain; separation of peptides by cation exchange HPLC; amino acid analysis; sequencing
DNA ANALYSIS Not reported; presumed mutation GAT->AAT at codon 99
NOTES Found in an Indian family; occurred in association with beta-thal; Hb X has an increased affinity for oxygen
1. Salkie, M.L., Gordon, P.A., Rigal, W.M., Lam, H., Wilson, J.B., Headlee, M.E., and Huisman, T.H.J.: Hemoglobin, 6:223, 1982.

This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.