Hb A2-Babinga delta136(H14)Gly->Asp
CONTACT Central cavity
ELECTROPHORESIS Hb X moves slightly faster than Hb A2 at alkaline pH
IEF Not reported
CHROMATOGRAPHY Hb X separates from Hb A2 on an anion exchange column
STRUCTURE ANALYSIS Tryptic digestion of AE-delta chain; separation of peptides by fingerprinting or cation exchange HPLC; amino acid analysis; sequencing
DNA ANALYSIS Not reported; presumed mutation GGT->GAT at codon 136
NOTES Found in Babinga Pygmies (frequency 1-2%) and also in a few Black families
1. De Jong, W.W.W. and Bernini, L.F.: Nature, 219:1360, 1968.
2. Huisman, T.H.J., Reynolds, C.A., Dozy, A.M., and Wilson, J.B.: Biochim. Biophys. Acta, 175:223, 1969.
3. McRoyan, D.K., Liu, P.I., Mankad, V.N., Wilson, J.B., and Huisman, T.H.J.: Hemoglobin, 8:413, 1984.

This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.