Hb Olomouc beta86(F2)Ala->Asp
CONTACT External
HEMATOLOGY Mild erythrocytosis in the heterozygote
ELECTROPHORESIS Hb X and Hb A separate at alkaline pH; Hb X moves faster than Hb A; excellent separation by IEF; Hb X moves between Hb A and Hb F at acidic pH
CHROMATOGRAPHY Hb X was isolated by DEAE-cellulose chromatography; Hb X elutes faster than Hb A on cation exchange HPLC columns
STRUCTURE STUDIES Tryptic digestion of AE-betaX chain; separation of tryptic peptides by reversed phase HPLC; amino acid analysis; sequencing
DNA ANALYSES Not reported; presumed mutation GCC->GAC at codon 86
FUNCTION STUDIES Slightly increased oxygen affinity
OCCURRENCE Found in members of a Czechoslovakian family and a Japanese family
OTHER INFORMATION Quantity in the heterozygote 46-47%
1. Indrak, K., Wiedermann, B.F., Batek, F., Wilson, J.B., Webber, B.B., Kutlar, A., and Huisman, T.H.J.: Hemoglobin, 11:151, 1987.
2. Tagawa, Y., Fujinami, S., Kadota, Y., Nakagawa, T., Seki, T., Shiozaki, Y., Harano, T., Harano, K., and Ueda, S.: Hemoglobin, 16:73, 1992.

This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.