Hb D-Los Angeles beta121(GH4)Glu->Gln
ALSO KNOWN AS D-Punjab; D-North Carolina; D-Portugal; Oak Ridge; D-Chicago
CONTACT External
HEMATOLOGY Normal in heterozygotes and homozygotes
ELECTROPHORESIS Hb X moves slower (like Hb S) than Hb A at alkaline pH; no separation at acidic pH
CHROMATOGRAPHY Hb X and Hb A separate by both cation and anion exchange chromatography
STRUCTURE STUDIES Tryptic digestion of AE-betaX chain; separation of peptides by fingerprinting or cation exchange chromatography or reversed phase HPLC; amino acid analysis; sequencing
DNA ANALYSES A GAA->CAA mutation at codon 121
OCCURRENCE Hb D-Los Angeles is primarily found in Northern India but is wide-spread and has been observed in persons from China, England, Holland, Australia, Greece, Yugoslavia, Turkey, etc. It is the fourth most frequently occurring Hb variant
OTHER INFORMATION Quantity in the heterozygote ~40%; it has been found in combination with Hb S, Hb C, Hb E, beta-thal, alpha-thal, and in the homozygous state
1. Itano, H.: Proc. Natl. Acad. Sci. USA, 37:775, 1951.
2. Hynes, M. and Lehmann, H.: Br. Med. J., 2:923, 1956.
3. Baglioni, C.: Biochim. Biophys. Acta, 59:437, 1969.
4. Bunn, H.F. and Forget, B.G.: Hemoglobin: Molecular, Genetic and Clinical Aspects, W.B. Saunders Company, Philadelphia, 1986.
5. Huisman, T.H.J., editor: The Hemoglobinopathies, Methods in Hematology, Vol. 15, Churchill Livingstone, Edinburgh, 1986.
6. Weatherall, D.J. and Clegg, J.B., editors: The Haemoglobinopathies, Bailliere's Clinical Haematology, Vol. 6, W.B. Saunders Company, London, 1993.

This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.