Hb Chico	beta66(E10)Lys->Thr

CONTACT		Heme contact
HEMATOLOGY		Mild anemia in the heterozygote
ELECTROPHORESIS		Hb X and Hb A separate at alkaline pH (Hb X has the mobility of Hb J) but not at acidic pH
CHROMATOGRAPHY		Incomplete separation of Hb X and Hb A by cation exchange HPLC; good separation by DEAE-Sephadex chromatography; betaA, betaX, and alpha chains separate in reversed phase HPLC; elution in the order listed
STRUCTURE STUDIES		Tryptic digestion of betaX chain; separation of peptides by reversed phase HPLC; amino acid analysis
DNA ANALYSES		Not reported; presumed mutation AAA->ACA at codon 66
FUNCTION STUDIES		Decreased oxygen affinity; normal cooperativity
STABILITY		Slightly unstable
OCCURRENCE		Found in a family living in Chico, California, USA
OTHER INFORMATION		Quantity in the heterozygote ~45%

REFERENCES
1.		Shih, D.T-B., Jones, R.T., Shih, M.F-C., Jones, M.B., Koler, R.D., and Howard, J.: Hemoglobin, 11:453, 1987.

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This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.