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CONTACT | |
C-terminus | |
HEMATOLOGY | |
Normal in the heterozygote | |
ELECTROPHORESIS | |
Hb X moves to the position of Hb J on cellulose acetate at alkaline pH; has a pI of 6.91 on IEF (Hb A 6.95) | |
CHROMATOGRAPHY | |
Hb X was isolated by anion exchange HPLC | |
STRUCTURE STUDIES | |
Tryptic digestion; separation of peptides by fingerprinting or cation exchange chromatography; amino acid analysis | |
DNA ANALYSES | |
Not reported; presumed mutation CGT->CAT; alpha2 or alpha1 | |
FUNCTIONAL STUDIES | |
Increased oxygen affinity; decreased cooperativity and Bohr effect | |
STABILITY | |
Not reported | |
OCCURRENCE | |
Found in a French and a Black family | |
OTHER INFORMATION | |
Quantity in heterozygotes 22-25% | |
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REFERENCES |
1. | |
Poyart, C., Krishnamoorthy, R., Bursaux, E., Gacon, G., and Labie, D.: FEBS Lett., 69:103, 1976. | |
2. | |
Bursaux, E., Krishnamoorthy, R., Poyart, C., and Bohn, B.: INSERM, 70:277, 1977. | |
3. | |
Poyart, C., Bursaux, E., Arnone, A., Bonaventura, J., and Bonaventura, C.: J. Biol. Chem., 255:9465, 1980. | |
4. | |
Gravely, M.E., Harris, H.F., Stallings, M., Lam, H., Wilson, J.B., and Huisman, T.H.J.: Hemoglobin, 2:187, 1978. | |