Hb O-Indonesia alpha116(GH4)Glu->Lys
ALSO KNOWN AS Buginese-X; Oliviere
CONTACT External
HEMATOLOGY Normal in the heterozygote
ELECTROPHORESIS Hb X moves slightly slower than Hb S at alkaline pH; Hb X moves as a sharp band close to but between Hb A and Hb S on citrate agar at acidic pH; excellent separation by IEF
CHROMATOGRAPHY Hb X was isolated by cation and anion exchange HPLC
STRUCTURE STUDIES Tryptic digestion; separation of peptides by fingerprinting; amino acid analysis
DNA ANALYSES A GAG->AAG mutation at codon 116 of the alpha1 gene (Ref. 3)
STABILITY Slightly less stable than Hb A
OCCURRENCE Found in Buginese from Sulawesi (formerly Celebes), Makassars, Iranian, South African, Italian, and Chinese families
OTHER INFORMATION Quantity in heterozygotes 21%; found in association with Hb D-Punjab and with beta-thal
1. Lie-Injo, L.E.: The Lancet, ii:1338, 1957.
2. Baglioni, C. and Lehmann, H.: Nature, 196:229, 1962.
3. Molchanova, T.P., Pobedimskaya, D.D., and Huisman, T.H.J.: Br. J. Haematol., 88:300, 1994.

This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.