Hb Kurosaki | alpha7(A5)Lys->Glu |
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CONTACT | External | ||
HEMATOLOGY | Normal in the heterozygote | ||
ELECTROPHORESIS | IEF demonstrated the presence of Hb X as a fast-moving band that focused anodally to Hb A (almost at the position of Hb H) | ||
CHROMATOGRAPHY | Fast-eluting Hb by cation exchange HPLC; Hb X and Hb A were also separated on a DEAE-5PW HPLC column | ||
STRUCTURE STUDIES | The alpha chain was isolated by CM-cellulose 8 M-urea-phosphate chromatography; tryptic peptides were separated by reversed phase HPLC; an alphaT-1,2 peptide was identified (alphaT-1 and alphaT-2 were absent) by amino acid analysis; sequence analysis | ||
DNA ANALYSES | Not reported; presumed mutation AAG->GAG; alpha2 or alpha1 | ||
FUNCTIONAL STUDIES | Normal | ||
STABILITY | Normal | ||
OCCURRENCE | Found in a 70-year-old diabetic Japanese female | ||
OTHER INFORMATION | Quantity in the heterozygote 17.7% |
REFERENCES | |||
1. | Harano, T., Harano, K., Imai, K., Murakami, T., and Matsubara, H.: Hemoglobin, 19:197, 1995. |