Hb F-Calluna Agamma12(A9)Thr->Arg
ELECTROPHORESIS Hb FX moves between Hb S and Hb C at alkaline pH
IEF Not reported
CHROMATOGRAPHY Hb FX elutes between Hb A2 and Hb A on a DEAE-cellulose column
CHAIN SEPARATION AgammaX = 10.7%; Ggamma = 71.5%; AgammaT = 17.8%
STRUCTURE STUDIES Tryptic digestion of Hb FX; separation of peptides by reversed phase HPLC; amino acid analysis
DNA ANALYSES Not reported; presumed mutation ACA->AGA at codon 12
NOTES Found in a Caucasian baby; Hb FX 8.7% of total Hb or 10.8% of total Hb F; baby was also heterozygous for the AgammaT chain
1. Nakatsuji, T., Lam, H., and Huisman, T.H.J.: Hemoglobin, 7:563, 1983.

This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.