Hb Natal alpha140(HC2)-alpha141(HC3) Tyr-Arg->0
HEMATOLOGY Modest erythrocytosis (PCV 0.55-0.56 l/l)
ELECTROPHORESIS Hb X moves faster than Hb A at alkaline pH and in IEF
CHROMATOGRAPHY Hb X elutes ahead of Hb A from a cation exchange HPLC column
STRUCTURE STUDIES Tryptic digestion of alphaX chain; separation of peptides by reversed phase HPLC; amino acid analysis; acid hydrolysis
DNA ANALYSES A TAC->TAA mutation at codon 140 of the alpha2 gene
FUNCTION STUDIES Increased oxygen affinity; no Bohr effect; no heme-heme interaction
OCCURRENCE Found in an Asian Indian and his father
1. Jogessar, V.B., Westermeyer, K., Webber, B.B., Wilson, J.B., Hu, H., Gonzalez-Redondo, J.M., Kutlar, A., and Huisman, T.H.J.: Biochim. Biophys. Acta, 951:36, 1988.

This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.