Hb F-Forest Park Agamma73(E17)Asp->Asn; 75(E19)Ile->Thr
ELECTROPHORESIS Hb FX moves between Hb S and Hb C at alkaline pH
IEF Not reported
CHROMATOGRAPHY Hb FX eluted between Hb A2 and Hb A in DEAE-cellulose chromatography
CHAIN SEPARATION AgammaX = 18.3%; Ggamma = 70.3%; Agamma = 11.4%
STRUCTURE STUDIES Tryptic digestion of gammaX chain; separation of peptides by reversed phase HPLC; amino acid analysis; sequencing
DNA ANALYSES A GAT->AAT mutation at codon 73; confirmed by gene mapping
NOTES Found in Caucasian newborn babies; Hb FX 12.2% of total Hb
1. Chen, S-S., Webber, B.B., Wilson, J.B., and Huisman, T.H.J.: Biochim. Biophys. Acta, 832:242, 1985.

This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.