Hb Birmingham Deletion of Leu-Ala-His-Lys- at beta141,
beta142, beta143, beta144; insertion of Gln-
HEMATOLOGY Hemolytic anemia; reticulocytosis; Heinz bodies
ELECTROPHORESIS Hb X moves about like Hb S in cellulose acetate electrophoresis at alkaline pH
CHROMATOGRAPHY The betaX chain elutes in two minor zones faster than the betaA chain (reversed phase HPLC)
STRUCTURE STUDIES Tryptic digestion of betaX chain; separation of peptides by reversed phase HPLC; amino acid analysis; sequencing
DNA ANALYSES Deletion of TG*GCC*CAC*A from codons 141 through 144 removes four amino acid residues (Leu, Ala, His, Lys); the remaining triplet CAG codes for glutamine
OCCURRENCE Found in an adult Black male
OTHER INFORMATION Quantity in the heterozygote ~20%
1. Wilson, J.B., Webber, B.B., Hu, H., Kutlar, A., Kutlar, F., Codrington, J.F., Prchal, J.T., Hall, K.M., de Pablos, J.Ma., Rodriguez, I., and Huisman, T.H.J.: Blood, 75: 1883, 1990.

This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.