Hb F-Catalonia | Ggamma15(A12)Trp->Arg |
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ELECTROPHORESIS | Hb FX moves slower than Hb F (between Hb S and Hb C) at alkaline pH | ||
IEF | Hb FX focuses between Hb S and Hb C | ||
CHROMATOGRAPHY | Hb FX can readily be separated from Hb A and Hb F by DEAE-cellulose chromatography | ||
CHAIN SEPARATION | GgammaX = 36.8%; Ggamma = 35.4%; Agamma = 27.8%; also see NOTES | ||
STRUCTURE STUDIES | Tryptic digestion of gammaX chain; separation of peptides by reversed phase HPLC; amino acid analysis | ||
DNA ANALYSES | Not reported; presumed mutation TGG->CGG at codon 15 | ||
NOTES | Found in two newborn babies from Northern Spain; the sec-ond baby was also an AgammaT heterozygote (Agamma75 Ile->Thr): AgammaT 11.5%; GgammaX 45.5%; Ggamma 31.3%; Agamma 11.7% |
REFERENCES | |||
1. | Plaseska, D., Wilson, J.B., Kutlar, F., Font, Ll., Baiget, M., and Huisman, T.H.J.: Hemoglobin, 14:511, 1990. |