Hb A2-Manzanares	delta121(GH4)Glu->Val

CONTACT		External
ELECTROPHORESIS		Hb X moves slower than Hb A2, about like Hb A2' (delta16)
IEF		About the same
CHROMATOGRAPHY		Hb X was isolated by DEAE-cellulose chromatography; Hb X is the first Hb fraction that is eluted
STRUCTURE ANALYSIS		Tryptic digestion of AE-deltaX chain; separation of peptides by reversed phase HPLC; amino acid analysis; sequencing
DNA ANALYSIS		Not reported; presumed mutation GAA->GTA at codon 121
NOTES		Found in a Spanish family; variant is rather unstable

REFERENCES
1.		Romero Garcia, C., Navarro, J.L., Lam, H., Webber, B.B., Headlee, M.G., Wilson, J.B., and Huisman, T.H.J.: Hemoglobin, 7:435, 1983.

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This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.