Hb North Chicago beta36(C2)Pro->Ser
CONTACT alpha1beta2 contact
HEMATOLOGY Erythrocytosis in the heterozygote
ELECTROPHORESIS No separation of Hb X and Hb A at alkaline pH; separation is possible by IEF; excellent separation of betaX, betaA, and alpha by PAGE
CHROMATOGRAPHY No separation reported; the betaX chain elutes after the normal alpha chain from a reversed phase HPLC column
STRUCTURE STUDIES Tryptic digestion of the betaX chain; separation of the peptides by fingerprinting and by reversed phase HPLC; amino acid analysis; ion mass spectral analysis
DNA ANALYSES Not reported; presumed mutation CCT->TCT at codon 36
FUNCTION STUDIES Increased oxygen affinity
OCCURRENCE Discovered in members of a Black family
1. Rahbar, S., Louis, J., Lee, T., and Asmerom, Y.: Hemoglobin, 9:559, 1985.

This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.