Hb F-Sardinia (AgammaT) Agamma75(E19)Ile->Thr
ELECTROPHORESIS No separation reported
IEF (Nearly) no separation
CHAIN SEPARATION AgammaT = ~15%; Ggamma = ~70%; AgammaI = ~15%
STRUCTURE STUDIES Tryptic digestion of AE-gammaX chain; separation of peptides by fingerprinting or reversed phase HPLC; amino acid analysis; sequencing
DNA ANALYSES An ATA->ACA mutation at codon 75
NOTES Found at high frequencies in nearly all populations; quantity in heterozygotes ~15% of total Hb F
1. Grifoni, V., Kamuzora, H., Lehmann, H., and Charlesworth, D.: Acta Haematol., 53:347, 1975.
2. Huisman, T.H.J., Schroeder, W.A., Reese, A., Wilson, J.B., Lam, H., Shelton, J.R., Shelton, J.B., and Baker, S.: Pediatr. Res., 11:1102, 1977.
3. Huisman, T.H.J., Kutlar, F., Nakatsuji, T., Bruce-Tagoe, A., Kilinç, Y., Cauchi, M.N., and Romero Garcia, C.: Hum. Genet., 71:127, 1985.

This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.